dapi staining solution (Beyotime)
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dapi staining solution
Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+with+dapi/DAPI+Staining+Solution/pmc13097096-64-0-21
Average 99 stars, based on 2029 article reviews
Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+with+dapi/DAPI+Staining+Solution/pmc13097096-64-0-21
Average 99 stars, based on 2029 article reviews
dapi staining solution - by Bioz Stars,
2026-09
99/100 stars
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Staining:Article Title: α -Ketoglutarate protects against cartilage damage via epigenetically driven metabolic reprogramming in osteoarthritis models Article Snippet: Immunofluorescence staining was carried out by incubation overnight at 4°C with antibodies against COL1A1 (Servicebio, GB11022-1; 1:200), Aspn (Affinity, DF13642; 1:100), Cilp (Huabio, ER1906-32; 1:100), Clu (Proteintech, 66109-1-Ig; 1:100), and Cnmd (Affinity, DF13715; 1:100). .. Alexa Fluor 594– or Cy3-conjugated secondary antibodies (Invitrogen) were added for 30 minutes, followed by Article Title: Exploring the role of RPS2 in muscle-invasive bladder cancer progression and resistance: A nanovesicle-based therapeutic approach Article Snippet: Newly synthesized RNA was labeled using the Cell-Light EU RNA Imaging Kit (C10316; RiboBio, Guangzhou, China). .. Cells were incubated with EU for 3h, followed by nuclear Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism Article Snippet: After blocking in 3 % BSA for half an hour, osteoblasts were incubated with a primary antibody anti -LC3 (1:500, 14600-1-AP, Proteintech, USA) at 4 °C overnight. .. Afterward, incubation with a secondary antibody Goat Anti-Rabbit Alexa Flour 488 (1:300, ab150077, Abcam, UK) was conducted at room temperature for 1 h. After Article Title: The HKDC1-ASS1-ACSBG2 axis reprograms lipid metabolism to drive therapeutic resistance in hepatocellular carcinoma Article Snippet: .. After three washes with PBST, the cells were incubated with fluorescent secondary antibodies (Beyotime, Shanghai, China) for 1 h, followed by nuclear Article Title: ROS-responsive Prussian blue nanoplatform for synergistic myocardial ischemia/reperfusion injury therapy via EGCG delivery and DJ-1 pathway regulation. Article Snippet: Effective management of myocardial ischemia/reperfusion (I/R) injury remains a clinical challenge.. Epigallocatechin gallate (EGCG), a natural antioxidant, exhibits therapeutic potential in I/R models; however, it is limited by poor bioavailability and inadequate targeted delivery.. Therefore, using a synergistic therapeutic strategy, we designed a nanoplatform (PB@PEI@FPBA-EGCG NPs, referred to as PPFE NPs) to address these limitations. Article Title: Dihydrolipoic acid suppresses ferroptosis in chondrocytes to ameliorate the progression of osteoarthritis by modulating the FOXO1/TXNIP signaling pathway. Article Snippet: .. On the second day, after 1 h of incubation with secondary antibodies in the dark at room temperature, the cells were then subjected to nuclear Article Title: After Traumatic Brain Injury, EPHA4 Enhances Endoplasmic Reticulum Stress to Promote M1 Microglial Polarization Through the MAPK Signaling Pathway Article Snippet: After the cell culture was complete, the medium was discarded, and the cells were fixed with 4% paraformaldehyde for 20 min. Then, the cells were incubated with primary antibodies against iNOS (1:200; 22226‐1‐AP; Proteintech, China) and Arg1 (1:200; 16001‐1‐AP; Proteintech, China) at 4°C overnight. .. Afterward, the corresponding fluorescent secondary antibody was incubated at room temperature for 2 h. After nuclear Article Title: Combined strategy of dual-module cerium nanosystem composite extracellular vesicles regulate ROS in the tissue microenvironment to promote periodontitis recovery Article Snippet: .. Cells were incubated with Vari Fluor 594-Phalloidin or Vari Fluor 488-Phalloidin (MCE) in the dark for 20 min, followed by nuclear Incubation:Article Title: Exploring the role of RPS2 in muscle-invasive bladder cancer progression and resistance: A nanovesicle-based therapeutic approach Article Snippet: Newly synthesized RNA was labeled using the Cell-Light EU RNA Imaging Kit (C10316; RiboBio, Guangzhou, China). .. Cells were incubated with EU for 3h, followed by nuclear Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism Article Snippet: After blocking in 3 % BSA for half an hour, osteoblasts were incubated with a primary antibody anti -LC3 (1:500, 14600-1-AP, Proteintech, USA) at 4 °C overnight. .. Afterward, incubation with a secondary antibody Goat Anti-Rabbit Alexa Flour 488 (1:300, ab150077, Abcam, UK) was conducted at room temperature for 1 h. After Article Title: The HKDC1-ASS1-ACSBG2 axis reprograms lipid metabolism to drive therapeutic resistance in hepatocellular carcinoma Article Snippet: .. After three washes with PBST, the cells were incubated with fluorescent secondary antibodies (Beyotime, Shanghai, China) for 1 h, followed by nuclear Article Title: ROS-responsive Prussian blue nanoplatform for synergistic myocardial ischemia/reperfusion injury therapy via EGCG delivery and DJ-1 pathway regulation. Article Snippet: Effective management of myocardial ischemia/reperfusion (I/R) injury remains a clinical challenge.. Epigallocatechin gallate (EGCG), a natural antioxidant, exhibits therapeutic potential in I/R models; however, it is limited by poor bioavailability and inadequate targeted delivery.. Therefore, using a synergistic therapeutic strategy, we designed a nanoplatform (PB@PEI@FPBA-EGCG NPs, referred to as PPFE NPs) to address these limitations. Article Title: Dihydrolipoic acid suppresses ferroptosis in chondrocytes to ameliorate the progression of osteoarthritis by modulating the FOXO1/TXNIP signaling pathway. Article Snippet: .. On the second day, after 1 h of incubation with secondary antibodies in the dark at room temperature, the cells were then subjected to nuclear Article Title: After Traumatic Brain Injury, EPHA4 Enhances Endoplasmic Reticulum Stress to Promote M1 Microglial Polarization Through the MAPK Signaling Pathway Article Snippet: After the cell culture was complete, the medium was discarded, and the cells were fixed with 4% paraformaldehyde for 20 min. Then, the cells were incubated with primary antibodies against iNOS (1:200; 22226‐1‐AP; Proteintech, China) and Arg1 (1:200; 16001‐1‐AP; Proteintech, China) at 4°C overnight. .. Afterward, the corresponding fluorescent secondary antibody was incubated at room temperature for 2 h. After nuclear Article Title: Combined strategy of dual-module cerium nanosystem composite extracellular vesicles regulate ROS in the tissue microenvironment to promote periodontitis recovery Article Snippet: .. Cells were incubated with Vari Fluor 594-Phalloidin or Vari Fluor 488-Phalloidin (MCE) in the dark for 20 min, followed by nuclear Fluorescence:Article Title: Exploring the role of RPS2 in muscle-invasive bladder cancer progression and resistance: A nanovesicle-based therapeutic approach Article Snippet: Newly synthesized RNA was labeled using the Cell-Light EU RNA Imaging Kit (C10316; RiboBio, Guangzhou, China). .. Cells were incubated with EU for 3h, followed by nuclear Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism Article Snippet: After blocking in 3 % BSA for half an hour, osteoblasts were incubated with a primary antibody anti -LC3 (1:500, 14600-1-AP, Proteintech, USA) at 4 °C overnight. .. Afterward, incubation with a secondary antibody Goat Anti-Rabbit Alexa Flour 488 (1:300, ab150077, Abcam, UK) was conducted at room temperature for 1 h. After Article Title: After Traumatic Brain Injury, EPHA4 Enhances Endoplasmic Reticulum Stress to Promote M1 Microglial Polarization Through the MAPK Signaling Pathway Article Snippet: After the cell culture was complete, the medium was discarded, and the cells were fixed with 4% paraformaldehyde for 20 min. Then, the cells were incubated with primary antibodies against iNOS (1:200; 22226‐1‐AP; Proteintech, China) and Arg1 (1:200; 16001‐1‐AP; Proteintech, China) at 4°C overnight. .. Afterward, the corresponding fluorescent secondary antibody was incubated at room temperature for 2 h. After nuclear Microscopy:Article Title: Exploring the role of RPS2 in muscle-invasive bladder cancer progression and resistance: A nanovesicle-based therapeutic approach Article Snippet: Newly synthesized RNA was labeled using the Cell-Light EU RNA Imaging Kit (C10316; RiboBio, Guangzhou, China). .. Cells were incubated with EU for 3h, followed by nuclear Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism Article Snippet: After blocking in 3 % BSA for half an hour, osteoblasts were incubated with a primary antibody anti -LC3 (1:500, 14600-1-AP, Proteintech, USA) at 4 °C overnight. .. Afterward, incubation with a secondary antibody Goat Anti-Rabbit Alexa Flour 488 (1:300, ab150077, Abcam, UK) was conducted at room temperature for 1 h. After Article Title: After Traumatic Brain Injury, EPHA4 Enhances Endoplasmic Reticulum Stress to Promote M1 Microglial Polarization Through the MAPK Signaling Pathway Article Snippet: After the cell culture was complete, the medium was discarded, and the cells were fixed with 4% paraformaldehyde for 20 min. Then, the cells were incubated with primary antibodies against iNOS (1:200; 22226‐1‐AP; Proteintech, China) and Arg1 (1:200; 16001‐1‐AP; Proteintech, China) at 4°C overnight. .. Afterward, the corresponding fluorescent secondary antibody was incubated at room temperature for 2 h. After nuclear Laser-Scanning Microscopy:Article Title: Combined strategy of dual-module cerium nanosystem composite extracellular vesicles regulate ROS in the tissue microenvironment to promote periodontitis recovery Article Snippet: .. Cells were incubated with Vari Fluor 594-Phalloidin or Vari Fluor 488-Phalloidin (MCE) in the dark for 20 min, followed by nuclear Confocal Laser Scanning Microscopy:Article Title: Combined strategy of dual-module cerium nanosystem composite extracellular vesicles regulate ROS in the tissue microenvironment to promote periodontitis recovery Article Snippet: .. Cells were incubated with Vari Fluor 594-Phalloidin or Vari Fluor 488-Phalloidin (MCE) in the dark for 20 min, followed by nuclear |
